By Physician’s Weekly –
Researchers conducted a retrospective study to analyze miRNA-mRNA regulatory networks influencing bone marrow mesenchymal stem cell (BMSCs) senescence in age-related osteoporosis (ARO), aiming to determine molecular markers and therapeutic targets.
They analyzed 5 mRNA datasets to identify differentially expressed genes linked to senescence and osteoporosis, 7 hub genes were enriched in the PI3K-Akt signaling pathway, with 22 hub miRNAs identified as potential regulators. The Primary BMSCs were harvested and cultured from 7 younger non-osteoporotic individuals and 6 older adults (OAs) with osteoporosis. Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to validate the expression levels of the hub genes and miRNAs.
