By Inside Precision Medicine –
U.S. researchers have shone further light into the precise way that CRISPR/Cas9 gene editing actually works and circumvents unintended off-target effects, which could lead to more accurate cellular therapies.
The preclinical research, published in the journal Cell Chemical Biology, outlines several steps that are necessary for the Cas9 complex to become active and allow it to accurately edit genes.
Specifically, the findings reveal a molecular mechanism by which on- and off-target DNA sequences are identified relative to the guide RNA template.
